| 摘要 |
Protein molecules (4a-4c, 10, 10a-10c) of interest are isolated and modified into linearized protein molecules (10, 10a-10c). Individual protein molecule s are isolated for observation with hydrodynamic focusing apparatus (130), atomic force microscope (150), a separation plate (170), or the combination of isoelectric focusing gel (180), and electrophoresis gel (184). In the linearized protein molecule (10, 10a-10c) a first type of amino acid residue (K) is labelled with a first tag (12), and a second type of amino acid resid ue (C) is labelled with a second tag (14). Tags (12, 14) impart a detectable se t of distinguishing characteristic ancillary properties (24, 34, 38, 44, 48, 5 2, 64, 68, 72, 120, 122, 206, 208, 210, 244, 246, 248) to the linearized protei n molecule (10, 10a-10c) that define the fingerprint thereof. Protein fingerprints are recovered as images (206, 208, 210, 244, 246, 248) by camer a (204) or fluorescence microscope (230), or as spectral analyses (24, 34, 38, 44, 48, 52, 64, 68, 72, 120, 122) by optical detector (15). Compilations of fingerprints for known protein molecules comprise fingerprint libraries to which the fingerprint of a protein molecule of interest is compared.
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