| 摘要 |
This invention describes novel charged molecules which specifically bind to the Hepreceptor, a regulatory site which I have discovered in human ezrin. M y invention is that when peptides or other charged molecules bind to the Hepreceptor, medically useful immune responses are induced. These charged molecules can be administered orally and by other routes for the treatment o f various infectious diseases and cancer. I have determined that the Heprecept or (human ezrin 308-373) comprises of two adjacent alpha helical domains which are folded together at a hinge region (M339-M340) and stabilised by complimentary side chain charges of the primary amino acid sequence in the soluble cytoplasmic conformation of ezrin. I have determined that in the unfolded membrane associated conformation of ezrin, the Hepreceptor is pushe d through the cell membrane and is exposed on the outer surface of the cell. Hepreceptor-Domain A (amino acid numbers 308-339 of human ezrin), comprises of the following 32 amino acid sequence. SEQ ID 1 A R E E K H Q K Q L E R Q Q L E T E K K R R E T V E R E K E Q M Hepreceptor-Domain B (amino acid numbers 340 - 373 of human ezrin), comprises of the following 34 amino acid sequence (Tyrosine 353 [Y] may be phosphorylated to phosphotyrosine [Yp] in the membrane associated conformationof ezrin): SEQ ID 2 M R E K E E L M L R L Q D Y(p) E E K T K K A E R E L S E Q I Q R A L Q.
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