发明名称 |
THIOLATION OF PROTEINS FOR RADIONUCLIDE-BASED RADIOIMMUNODETECTION AND RADIOIMMUNOTHERAPY |
摘要 |
A method of radiolabeling a protein with a radionuclide including contacting the protein with a protected tertiary thiol-containing bifunctional chelatin g agent that is capable of reacting with the protein at one end of the agent a nd is capable of complexing with a radionuclide at the other end of the agent, to form a protein-acetyl-t-thiol-containing conjugate. The protein-acety l- t-thiol-containing conjugate then is deprotected and admixed with a reducing agent for the radionuclide, where the radionuclide is added in a subsequent step, to form a mixture of reducing agent and protein-t- thiol-containing conjugate. This mixture then is reacted with a radionuclide whereby the radionuclide reacts with pendant sulfhydryl groups present on th e protein-t-thiol-containing conjugate. Methods of radioimmunotherapy a nd diagnostic kits suitable for forming a composition to be administered to a human patient also are disclosed.
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申请公布号 |
CA2191858(C) |
申请公布日期 |
2001.04.10 |
申请号 |
CA19952191858 |
申请日期 |
1995.05.08 |
申请人 |
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发明人 |
GOVINDAN, SEREGULAM V.;GREBENAU, RUTH;GRIFFITHS, GARY L.;HANSEN, HANS J. |
分类号 |
A61K38/00;A61K39/395;A61K51/00;A61K51/08;A61K51/10;A61P9/08;A61P9/10;A61P31/00;A61P35/00;A61P37/00;C07B59/00;C07K1/13;C07K14/00;C07K16/00;C09K3/00;(IPC1-7):C07K1/13 |
主分类号 |
A61K38/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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