发明名称 |
Immortalized human keratinocyte cell line |
摘要 |
A spontaneously immortalized human keratinocyte cell line is disclosed. In a preferred embodiment, this cell line is ATCC 12191. In another embodiment of the invention, a method of assaying the effect of a test tumor cell modulation agent is disclosed. The method comprises the steps of obtaining a human stratified squamous epithelial cell culture, wherein the culture comprises human malignant squamous epithelial cells and spontaneously immortalized human keratinocytes, wherein the culture forms a reconstituted epidermis. One then treats the epidermis with a test tumor cell modulation agent and evaluates the growth of the malignant cells within the epidermis. |
申请公布号 |
US6214567(B1) |
申请公布日期 |
2001.04.10 |
申请号 |
US19990277295 |
申请日期 |
1999.03.26 |
申请人 |
WISCONSIN ALUMNI RESEARCH FOUNDATION |
发明人 |
ALLEN-HOFFMANN B. LYNN;PICKART MICHAEL A. |
分类号 |
A61P35/00;A61P43/00;C12N1/00;C12N5/071;C12N5/10;C12N15/09;C12Q1/02;G01N33/15;G01N33/50;(IPC1-7):G01N33/567;C12N5/08 |
主分类号 |
A61P35/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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