HIGH SENSITIVE IMMUNOASSAY

摘要

A time-resolved fluorescence immunoassay (TR-FIA) for detecting cytokine in a liquid biogenic sample, which comprises a step of capturing cytokine and forming its complex with a lanthanoid metal ion having a fluorescent moiety on a solid phase and a step of measuring the fluorescence from the fluorescent moiety, wherein the complex comprises (a) a first antibody having a moiety bound to the solid phase and a region capable of binding with cytokine, (b) cytokine, (c) a second antibody having a region capable of binding with cytokine and a moiety bound with biotin, (d) a connective having streptoavidin or avidin and a fluorescent moiety capable of forming a complex with a lanthanoid metal ion and (e) a lanthanoid metal ion, which are bound sequentially in this order. The flurescent moiety is represented by the general formula (I). The immunoassay can be used for detecting cytokine in a biogenetic liquid sample with improved sensitivity.