PROCESS FOR PRODUCING TRANSGLUTAMINASE

摘要

A process for the secretory production of a foreign protein (in particular, transglutaminase) by using a coryneform bacterium. Namely, a process for producing a foreign protein (in particular, transglutaminase) which comprises making a coryneform bacterium to produce an industrially useful foreign protein (in particular, transglutaminase) and efficiently releasing the product outside the cells (i.e., secretory production). A target foreign protein (in particular, transglutaminase) is produced by using an expression construct wherein the target foreign protein gene sequence containing the pro-structure part (in particular, pro-transglutaminase gene sequence) is ligated to the downstream of a sequence encoding the signal peptide domain originating in a coryneform bacterium, transferring this expression type gene construct into the coryneform bacterium, culturing the thus transformed coryneform bacterium, and treating the protein which is released outside the cells with a protease, etc. to thereby cleave and eliminate the pro-part.