| 摘要 |
An object of the invention is to provide simple, nontoxic, and pharmaceutically defined methods for genetic modification of cells and tissu es which enable development of a variety of molecular medicines. The invention herein is directed to a method for improving the transfer efficiency of biological material into cells and decreasing the frequency of cell death, i n vitro, in vivo, and ex vivo. Specifically, the invention involves the synergistic employment of biopolymer binding reagents, e.g. polynucleotide binding reagents, in combination with electroporation techniques to enhance the efficiency of polynucleotide transfer to the cells of interest and to decrease the frequency of cell death of these cells. The improved electroporation-based gene therapies find particular utility in the transfer of genetic material into cells and has broad applicability for a wide range of gene therapy and polynucleotide vaccine applications. The improved methods o f the present invention are useful with both ex vivo and in vivo delivery systems. In addition, the invention herein is not limited to the transport o f polynucleotides but rather, is also applicable to the transport of other biopolymers, such as proteins and antigens across cell membranes.
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