TRANSGENIC vWF AND vWF-RELATED POLYPEPTIDES, TRANSGENICS, METHODS, COMPOSITIONS, USES AND THE LIKE RELATING THERETO

摘要

The invention provides, among other things, transgenic vWF ("TrG-vWF") and vWF-related ("TrG-vWF*Rel") polypeptides and proteins, compositions comprising TrG-vWF and/or TrG-vWF*Rel or both, transgenic organisms for making TrG-vWF or TrG-vWF*Rel, methods for making the transgenic organisms, methods for making TrG-vWF and/or TrG-vWF*Rel-comprising compostions and for further purifying TrG-vWF or TrG-vWF*Rel- from the compositions. Illustrative embodiments of the invention particularly provide transgenic mammals that express an exogenous gene for vWF and excrete the vWF encoded by the gene into their milk. In a highly particular illustrative embodiment in this regard the invention provides transgenic female pigs that express vWF in their milk. In this regard, the invention relates particularly to female pigs having stably incorporated in their genomes a DNA comprising a region that encodes vWF operably linked to a mammary gland-specific promoter. Further in this regard the invention relates to the milk containing the TrG-vWF or TrG-vWF*Rel and to TrG-vWF and/or IrG-vWF*Rel containing compositions derived from the milk. It also relates to novel TrG-vWF or TrG-vWF*Rel polypeptides and proteins, and compositions comprising the same, obtained by expression of genes encoding vWF and vWF-related polypeptides in transgenic organisms. In addition to providing TrG-vWF or TrG-vWF*Rel, it relates to using TrG-vWF and TrG-vWF*Rel and to methods and compositions therefor.