New nucleic acid encoding bovine acetyl coenzyme A carboxylase alpha and its promoter, for milk-specific production of proteins and for regulating fat content of milk

摘要

A nucleic acid (I) comprising: (i) a sequence (S1) of 3690 base pairs (bp) or a sequence (S2) of 7255 bp; (ii) allelic variants of (i); or (iii) fragments of (i) or (ii) containing nucleotides (nt) 933-966, 2188-2219 or 3055-3495 of (S1), nt 1-441 of (S2), or the corresponding regions of their allelic variants, is new. Independent claims are also included for the following: (1) vectors that contain (I); (2) an expression vector containing a nucleic acid that includes the 2188-2219 nt region of (S1); (3) host cells containing a vector of (1) or (2); (4) a transgenic, non-human animal containing cells of (3); (5) producing a non-human transgenic mammal in which the milk has reduced fat content; and (6) genotyping cattle by analyzing the nt 933-966 region of (S1).