发明名称 |
THERMOSTABLE ENZYME PROMOTING THE FIDELITY OF THERMOSTABLE DNA POLYMERASES- FOR IMPROVEMENT OF NUCLEIC ACID SYNTHESIS AND AMPLIFICATION <i>IN VITRO</i> |
摘要 |
A purified thermostable enzyme is derived from the thermophilic archaebacterium <i>Archaeoglobus fulgidus</i>. The enzyme can be native or recombinant, is stable under PCR conditions and exhibits double strand specific exonuclease activity. It is a 3'-5' exonuclease and cleaves to produce 5'-mononucleotides. Thermostable exonucleases are useful in many recombinant DNA techniques, in combination with a thermostable DNA polymerase like <i>Tag</i> especially for nucleic acid amplification by the polymerase chain reaction (PCR).
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申请公布号 |
WO0123583(A2) |
申请公布日期 |
2001.04.05 |
申请号 |
WO2000EP09423 |
申请日期 |
2000.09.27 |
申请人 |
ROCHE DIAGNOSTICS GMBH;ANKENBAUER, WALTRAUD;LAUE, FRANK;SOBEK, HARALD;GREIF, MICHAEL |
发明人 |
ANKENBAUER, WALTRAUD;LAUE, FRANK;SOBEK, HARALD;GREIF, MICHAEL |
分类号 |
C12N15/09;C12N9/12;C12N9/16;C12N9/22;C12N15/10;C12N15/54;C12N15/55;C12P19/34;C12Q1/68;(IPC1-7):C12N15/55 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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地址 |
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