Chemiluminescent composition containing cationic surfactants or polymers and 4'hydroxyacetanilide, test kits and their use in analytical methods.

摘要

An aqueous composition useful for providing a chemiluminescent signal in analytical methods is stabilized by the presence of specific amounts of either a low molecular weight cationic surfactant or a cationic polymer. The composition also includes a 2,3-dihydro-1,4-phthalazinedione derivative, such as luminol, and 4'-hydroxyacetanilide. A chemiluminescent signal is generated using the composition in the presence of peroxidase or a peroxidase-labeled specific binding species (such as an antibody or oligonucleotide).