发明名称 |
Ribozyme-mediated synthesis of circular RNA |
摘要 |
Circular RNAs may be synthesized by inserting DNA fragments into a plasmid containing sequences having the capability of spontaneous cleavage and self-circularization. Insertion of the DNA fragments allows RNAs of predetermined size to be constructed. In addition, a two-dimensional polyacrylamide gel electrophoresis system having a second dimension more highly cross-linked than the first dimension permits the separation and analysis as well as the precise sizing of both linear and circular RNAs produced by the synthetic method.
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申请公布号 |
US6210931(B1) |
申请公布日期 |
2001.04.03 |
申请号 |
US19980200955 |
申请日期 |
1998.11.30 |
申请人 |
THE UNITED STATES OF AMERICA AS REPRESENTED BY THE SECRETARY OF AGRICULTURE |
发明人 |
FELDSTEIN PAUL A.;OWENS ROBERT A.;LEVY LAURENE;RANDLES JOHN W. |
分类号 |
C12N15/10;C12N15/113;C12P19/34;(IPC1-7):C12P19/34 |
主分类号 |
C12N15/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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