PREPARATION OF PROTEASE WHICH ACTIVATES BLOOD COAGULATION FACTOR VII, PROENZYME THEREOF, OR MIXTURE OF THEM IN PURE FORM BY AFFINITY CHROMATOGRAPHY

摘要

PROBLEM TO BE SOLVED: To provide a method for preparing protease which activates blood coagulation factor VII, proenzyme thereof, or a mixture of them in a pure form by the affinity chromatography. SOLUTION: This method comprises one affinity chromatographic step or more utilizing adsorption on calcium phosphate/hydroxyapatite, hydrophobic matrix, chelate matrix, matrix obtained by immobilizing a heparin-related substance such as heparin, heparan sulfate, and dextran sulfate, and/or immobilized monoclonal antibody or polyclonal antibody against a protein to be isolated, or matrix coated by its F(ab) or F(ab)2 fragment, and/or fractional precipitation.