Culture medium for insect cells lacking glutamine an contains ammonium salt

摘要

A culture medium for growth of insect cells, such as Spodoptera frugiperda, is provided. The culture medium lacks glutamine, and may optionally lack glutamate and/or aspartate. Further the insect culture medium contains an ammonium ion source, such as an ammonium salt. The culture medium is particularly useful for culturing the insect cells for a variety of purposes, for example, producing proteins or polypeptides using a baculovirus expression system.