Multiplex-PCR zum Nachweis von EHEC-Infektionen

摘要

The invention relates to a method for detecting clinically relevant EHEC infections by amplifying both stxA1 and stxA2 sequences in a multiplex amplification reaction. The inventive method is further characterized in that both sequences of human-pathogenic and sequences of porcine-pathogenic stxA2 isoforms are amplified with the primers derived from a specific region of the stxA2 gene.