| 摘要 |
<p>The invention concerns a method for identifying genes coding for cell constituents involved in the regulation of angiogenesis, characterised in that it comprises the following steps: (a) culturing endothelial cells on a protein of the extracellular matrix, according to four types of different conditions: a reference condition, a condition promoting angiogenesis, a condition inhibiting angiogenesis, a control condition; (b) isolating the messenger RNA derived from the cultured cells according to the different conditions; (c) comparing qualitatively and/or quantitatively different mRNA populations, so as to identify the mRNA's exclusively derived, or in particularly large amounts, from cell cultures under condition stimulating and/or inhibiting angiogenesis, corresponding to the genes coding for cell constituents involved in the regulation of angiogenesis. The invention also concerns a kit for implementing said method.</p> |
