PROCESS FOR MANUFACTURE OF 1-DEAMINO-8-D-ARGININE VASOPRESSIN

摘要

A process for the manufacture of 1-deamino-8-D-arginine vasopressin (DDAVP) comprising a preparation of Mpa(R1)-Tyr- Phe-Gln-Asn-Cys(R2)-Pro-OH (SEQ ID NO: 1), where R1 and R2 are sulfhydryl-pr otecting groups, with the dipeptide (R3)-D-Arg(HCl)-Gly- NH2, where R3 is an acid-sensitive amino-protecting group, to form Mpa(R1)-T yr-Phe-Gln-Asn-Cys(R2)-Pro-D-Arg(HCl)-Gly-NH2 (SEQ ID NO: 1), which is oxidized with iodine in a protic solvent. The reaction m ixture containing the oxidized product can be purified b y ion exchange chromatography on a cation exchange resin equilibrated with acid. A lso disclosed is high-purity DDAVP obtained by this proc ess and its use for treating diurea.