摘要 |
A method of linking a first molecule M<1>-NH2 with a second molecule M<2>-OH comprising reaction of a compound of the formula (I): M<1>-NH-CO-A-CO-SR<1> wherein M<1> is the residue of a molecule bearing an amino group, A is an alkylene or arylene group, R<1> is alkyl or aryl, with a compound of formula (II), M<2> is the residue of a molecule bearing a hydroxy group wherein B is a linker, D is C1-4 alkylene group or C3-12 arylene group, R<2> is hydrogen or a thiol protecting group. In addition, this invention relates to conjugate products of the coupling reaction, reagents for modifying M<1>-NH2 and M<2>-OH, and kits comprising these reagents. |