| 摘要 |
The present invention relates to methods of inducing an immune response against an antigen in a subject by administering to the subject, a therapeutically effective amount of a dendritic cell, or of a progenitor thereof, which is transduced with a replication defective pseudotyped lentiviral vector containing a nucleic acid sequence encoding a polypeptide, which is expressed in said dendritic cell and is presented on the surface of the dendritic cell, such that, following administration to a subject, an immune response is induced against the antigen in the subject. The invention also relates to a method of inducing an immune response against an antigen in a subject, by transducing a dendritic cell, or a progenitor of a progenitor thereof, with a pseudotyped lentiviral vector containing a nucleic acid sequence encoding polypeptide, which is expressed in the dendritic cell and is presented on the surface of the dendritic cell, and contacting the dendritic cell with a T cell to produce an activated T cell. The invention further provides a method of activating a T cell by contacting a T cell with a dendritic cell, or a progenitor of a dendritic cell, which expresses an antigen encoded by a transduced pseudotyped lentiviral vector, on its surface. The invention also relates to a vector, which contains an envelope protein from a virus other than a lentivirus, and a lentivirus genome encoding non-functional env and nef gene products; or a lentivirus genome encoding env and nef gene products and at least three accessory protein gene products, for example, non-functional vif, vpr and vpu. |
