| 发明名称 |
IMPROVEMENT OF CLAVULANIC ACID PRODUCTION |
| 摘要 |
The invention methods for the enhancement of clavulanic acid production. In particular, the invention provides a method for increasing the production of clavulanic acid by: gene dosage with <i>orf</i>2 from the clavulanic acid biosynthetic pathway in <i>Streptomyces clavuligerus</i>; and by manipulation of fermentation conditions, especially the concentration of DG3P, a substrate of N2(carboxyethyl)arginine synthase, the protein encoded by <i>orf</i>2. A method for preparing N2(carboxyethyl)arginine synthase is also provided, as is an assay for identifying its substrates.
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| 申请公布号 |
WO0119959(A1) |
申请公布日期 |
2001.03.22 |
| 申请号 |
WO2000US25186 |
申请日期 |
2000.09.15 |
| 申请人 |
THE JOHNS HOPKINS UNIVERSITY;TOWNSEND, CRAIG, A.;LI, RONGFENG;KHALEELI, NUSRAT |
发明人 |
TOWNSEND, CRAIG, A.;LI, RONGFENG;KHALEELI, NUSRAT |
| 分类号 |
C12N15/06;C12N15/16;C12N15/76;C12P17/18;(IPC1-7):C12N1/20;C12N15/00;C12N15/09;C12N15/63;C12N15/70;C12N15/74;C07H21/02;C07H21/04 |
主分类号 |
C12N15/06 |
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