GLUCOSE SENSING MOLECULES HAVING SELECTED FLUORESCENT PROPERTIES

摘要

An analyte sensing fluorescent molecule that employs intramolecular electron transfer is designed to exhibit selected fluorescent properties in the presence of analytes such as saccharides. The selected fluorescent propertie s include excitation wavelength, emission wavelength, fluorescence lifetime, quantum yield, photostability, solubility, and temperature or pH sensitivity . The compound comprises an aryl or a substituted phenyl boronic acid that act s as a substrate recognition component, a fluorescence switch component, and a fluorophore. The fluorophore and switch component are selected such that the value of the free energy for electron transfer is less than about 3.0 kcal m ol- 1. Fluorescent compounds are described that are excited at wavelengths great er than 400 nm and emit at wavelengths greater than 450 nm, which is advantageo us for optical transmission through skin. The fluorophore is typically selected from transition metal-ligand complexes and thiazine, oxazine, oxazone, or oxazine-one as well as anthracene compounds. The fluorescent compound can be immobilized in a glucose permeable biocompatible polymer matrix that is implantable below the skin.