A method for reverse transcribing RNA isolated in dry form

摘要

An RNA target is reverse transcribed by: (i) isolating nucleic acids from a sample to form a dry composition comprising the target RNA; and (ii) contacting the dry composition with a primer that will anneal to the RNA target, four different nucleoside triphosphates, and an enzyme having reverse transcriptase activity, under conditions such that the RNA target is reverse transcribed to form cDNA complementary to the RNA target.