Kojibiose phosphorylase glucosyl-saccharides produced by transglycosylation

摘要

A kojibiose phosphorylase which hydrolyzes kojibiose in the presence of an inorganic phosphoric acid to form D-glucose and beta-D-glucose-1-phosphoric acid, forms kojibiose and an inorganic phosphoric acid from beta-D-glucose-1-phosphoric acid, and catalyzes the transfer reaction of glucosyl group to other saccharides using beta-D-glucose-1-phosphoric acid as a saccharide donor. The enzyme is obtainable from natural sources such as microorganisms of the genus Thermoanaerobium, and obtainable by recombinant technology. Kojibiose phosphorylase is used to transfer glucose from glucose-1-phosphate to acceptor saccharides to produce alpha-D-glucosyl-1(1->5)-L-sorbose, kojibiosylglucose, kojibiosylglucoside and kojibiosylfructoside.