CCR5 RNA transcription based amplification assay

摘要

A method and a test kit for genotyping the CCR5 chemokine receptor. CCR5 transcript DNA is amplified using transcription based amplification. The amplification product is detected through the hybridization of appropriately labeled oligonucleotide wild type and mutant probes. In a liquid hybridization based ECL analysis, the mutant probe is a 16mer having the sequence 5'TCCATACATTAAAGAT3' [SEQ ID NO:9]. The method and kit can be used to predict.