Cell-free assay for plant gene targeting and conversion

摘要

The invention is based on the reaction of oligonucleotides in a cell-free system containing a plant cell extract and a test duplex DNA on a plasmid. The reaction specifically corrects or causes a mutation in a selectable marker gene to a form that can be selected in transformed MutS and RecA deficient bacteria. After transformation into MutS and RecA deficient bacteria, the gene conversion can be detected and quantified.