| 摘要 |
Methods for determining the presence of double stranded nucleic acids in a sample are provided. As illustrated in the figure, nucleic acids present in a fluid sample are translocated through a nanopore, e.g. by application of an electric field to the fluid sample. The current amplitude through the nanopo re is monitored during the translocation process and changes in the amplitude a re related to the passage of single- or double-stranded molecules through the nanopore. The subject methods find use in a variety of applications in which the detection of the presence of double-stranded nucleic acids in a sample i s desired, e.g. in hybridization assays, such as Northern blot assays, Souther n blot assays, and array based hybridization assays.
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