发明名称 |
AQUEOUS IMMUNOLOGIC ADJUVANT COMPOSITIONS OF MONOPHOSPHORYL LIPID A |
摘要 |
An aqueous adjuvant composition comprising an attenuated lipid A derivative and a non-immunostimulatory surfactant or surfactants enhances the immunological response in a warm blooded animal to a protein antigen. Attenuated lipid A derivatives useful according to the subject invention include monophosphoryl lipid A and 3-O-deacylated monophosphoryl lipid A. A surfactant or mixtures of surfactants are dissolved in a solvent. 1,2 Dipalmitoyl-sn-glycero-3-phosphocholine is a preferred surfactant. The dissolved surfactant is added to an attenuated lipid A derivative to obtain a mixture. The molar ratio of attenuated lipid A derivative to surfactant in the mixture is about 4:1. The solvent is evaporated and water is added to the resulting film. The suspension is sonicated in a 60 DEG C water bath until it becomes clear. Animals administered the adjuvant formulation exhibited increased antibody responses to a given antigen as well as displayed enhanced lymphocyte proliferative and cytotoxic T-lymphocyte responses. Intranasal administration of the aqueous adjuvant composition and an antigen stimulates the production of serum and mucosal secreted IgA.
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申请公布号 |
WO0078353(A3) |
申请公布日期 |
2001.03.15 |
申请号 |
WO2000US16384 |
申请日期 |
2000.06.14 |
申请人 |
CORIXA CORPORATION |
发明人 |
CRANE, R., THOMAS |
分类号 |
A61K9/08;A61K9/00;A61K9/127;A61K35/74;A61K39/04;A61K39/08;A61K39/29;A61K39/39;A61K47/10;A61K47/16;A61K47/18;A61K47/36;A61P37/04;C07H13/06;(IPC1-7):A61K39/39 |
主分类号 |
A61K9/08 |
代理机构 |
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