PARAMYXOVIRUSES COMPRISING MODIFIED TRANSCRIPTION START SEQUENCE

摘要

Virus vectors of the family Paramyxoviridae in which the transcription initiation sequence has been modified and thus the expression of genes locat ed downstream thereof has been modified; a process for producing the same; and use of the same. By measuring the transcription initiation efficiency of eac h gene carried by Sendai viruses, it is clarified that the transcription initiation sequence of F gene has a significantly lower ability to promote t he transcription than the three other transcription initiation sequences. When the transcription initiation sequence of the F gene of wild type Sendai viru s is substituted by the transcription initiation sequence of the P/M/HN genoty pe showing a high efficiency, the F gene of the resultant Sendai virus mutant a nd genes located downstream thereof show elevated expression doses. It is also found out that this mutant proliferates more quickly than the wild type. The above-described vectors are useful in producing medicinal compositions and vaccines.