A NOVEL TYPE II RESTRICTION ENDONUCLEASE, Hpy188I, OBTAINABLE FROM HELICOBACTER PYLORI J188 AND A PROCESS FOR PRODUCING THE SAME

摘要

In accordance with the present invention, there is provided a novel restriction endonuclease and its DNA obtainable from Helicobacter pylori J188 (NEB#1174), hereinafter referred to as 'Hpy188I', which endonuclease: (1) recognizes the nucleotide sequence 5'-TCNGA-3' in a double-stranded DNA molecule as shown below, 5'-TCN↓GA-3' 3'-AG↑NCT-5' (wherein G represents guanine, C represents cytosine, A represents adenine, T represents thymine and N represents either G, C, A, or T); (2) cleaves said sequence in the phosphodiester bonds between the N and G as indicated with the arrows; and (3) cleaves double-stranded PhiX174 DNA to produce 18 fragments, including fragments of 1331, 813, 572, 525, 396, 302, 293, 219 base pairs, and 10 fragments smaller than 200 base pairs.