OLIGONUCLEOTIDE N3'→P5' THIOPHOSPHORAMIDATES: THEIR SYNTHESIS AND USE

摘要

Oligonucleotides with a novel sugar-phosphate backbone containing at least one internucleoside 3'-NHP(O)(S-)O-5' linkage, and methods of synthesizing and using the inventive oligonucleotides are provided. The inventive thiophosphoramidate oligonucleotides were found to retain the high RNA binding affinity of the parent oligonucleotide N3'→P5' phosphoramidates and to exhibit a much higher acid stability.