Method and reagent for the detection or determination of HCV core antigens

摘要

The present invention provides an immunoassay for the detection of HCV core antigens in a sample which comprises subjecting an alkaline phosphatase-labeled anti-HCV core protein antibody and an HCV core antigen in the sample to antigen-antibody reaction in an aqueous solution containing a salt at a high concentration to form an antigen-antibody complex, and measuring the alkaline phosphatase activity of the formed antigen-antibody complex. Also provided is a reagent for the detection of HCV core antigens comprising an alkaline phosphatase-labeled anti-HCV core protein antibody, a salt at a concentration of 0.5 M to saturation and a reagent for the measurement of alkaline phosphatase activity.