发明名称 |
Chimeric dna/rna ribozymes containing propanediol |
摘要 |
DNA-RNA-(Pr)n-RNA-DNA ribozymes of formula III or IV:in whichX and Z comprise DNA sequences that base pair with an RNA substrate at positions adjacent to an RNA cleavage site;cugaugag and cgaaa are catalytic RNA sequences;Pr is a spacer residue -P(O)(OH)-O-CH2CH2CH2-O-; andR is a bridging residue -O-CH2-C(CH2OH)(CH3)-CH2-O-.The ribozymes can be made on any DNA synthesizer using phosphoramidite chemistry, and are useful as therapeutic agents for treating viral or endogenous RNA-mediated diseases. Preferred ribozymes target a sequence in the U5 region of HIV-1. |
申请公布号 |
AU6533200(A) |
申请公布日期 |
2001.03.13 |
申请号 |
AU20000065332 |
申请日期 |
2000.08.10 |
申请人 |
CITY OF HOPE |
发明人 |
JOHN J. ROSSI;PIOTR M SWIDERSKI |
分类号 |
C12N9/00;A61K31/7088;A61P7/02;A61P31/12;A61P43/00;C12N15/113 |
主分类号 |
C12N9/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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