SEQUENCE-SPECIFIC DNA RECOMBINATION IN EUKARYOTIC CELLS

摘要

The invention relates to a method for carrying out the sequence-specific recombination of DNA in eukaryotic cells comprising the following steps: inserting a first DNA sequence into a cell; inserting a second DNA sequence into a cell, and; carrying out the sequence-specific recombination using the action of a bacteriophage lambda integrase Int. A preferred embodiment of the invention relates to a method that additionally comprises the step of carrying out a second sequence-specific recombination of DNA using the action of an Int and of an Xis factor. The invention also relates to vectors and to their use as medicaments.