发明名称 |
ZYMOGEN ACTIVATION SYSTEM |
摘要 |
We describe the DNA sequences encoding an expression vector system that will permit, through limited proteolysis, the activation of expressed zymogen precursor of (S1) serine proteases in a highly controlled and reproducible fashion. The processed expressed protein, once activated, is rendered in a form amenable to measuring the catalytic activity. This catalytic activity of the activated form, is often a more accurate representation of the mature S1 protease gene product relative to the unprocessed zymogen precursor. Thus, this series of zymogen activation constructs represents a significant system for the analysis and characterization of serine protease gene products. |
申请公布号 |
WO0116289(A2) |
申请公布日期 |
2001.03.08 |
申请号 |
WO2000US22283 |
申请日期 |
2000.08.14 |
申请人 |
ORTHO-MCNEIL PHARMACEUTICAL, INC. |
发明人 |
DARROW, ANDREW;QI, JENSON;ANDRADE-GORDON, PATRICIA |
分类号 |
C12N15/09;A61K8/00;A61K8/66;A61K38/00;A61K38/48;A61K47/48;A61P15/00;A61P17/00;A61P25/00;A61P29/00;A61P43/00;A61Q5/02;C11D3/386;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12N9/50;C12N9/64;C12N15/57;C12N15/62;C12P21/06;C12Q1/37;G01N33/15;G01N33/50;G01N33/58 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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