STEREOSTRUCTURE OF DECARBAMYLASE AND METHOD OF USING THE SAME

摘要

Excellent decarbamylase variants which are more advantageously usable industrially are provided by clarifying the stereostructure of decarbamylase by X-ray crystalline structure analysis and designing molecules aiming at elevating the reactivity to the substrates (D-N-carbamoyl- alpha -amino acids), etc. by using the stereostructure. More particularly speaking, provision is made of: the stereostructure of decarbamylase determined by X-ray crystalline structure analysis, stereostructure models of variants thereof and stereostructure models of complexes thereof with substrates, products, etc.; a molecular design method with the use of these stereostructures; a method of obtaining decarbamylase variants by using this method; the decarbamylase variants obtained by this method; and a method of designing and producing variants of proteins which are similar to decarbamylase in structure.