PURIFICATION AND ANALYSIS OF CYCLIC PEPTIDE LIBRARIES, AND COMPOSITIONS THEREOF

摘要

Disclosed are methods of purifying and analyzing cyclic peptide libraries using hydrophobic-hydrophobic interaction. An embodiment of the invention includes associating a hydrophobic tag, e.g., a fluorenylmethoxycarbonyl group (Fmoc), with non-cyclized linear peptide contaminants, followed by separation of the cyclic peptide library from the tagged linear peptide contaminants using a hydrophobic support such as a reversed phase chromatography column. Consequently, the cyclic peptide library is separated from the linear peptide contaminants since the linear peptide contaminants elute later from the support. Another aspect of the invention is directed to the analysis of the proportion of linear peptide contaminants in a cyclic peptide library. Also included within the scope of the invention are the compositions of matter which result from practicing methods of the invention.