发明名称 |
L-sorbose dehydrogenase and novel L-sorbosone dehydrogenase obtained from gluconobacter oxydans T-100 |
摘要 |
A novel L-sorbose dehydrogenase (SDH) and a novel L-sorbosone dehydrogenase both derived from Gluconobacter oxydans T-100, a DNA which encodes the SDH and/or SNDH, an expression vector which contains the DNA, a host cell transformed by the expression vector and a process for producing the SDH and/or SNDH, which comprises culturing the host cell in a medium and recovering the SDH and/or SNDH from the resulting culture. The SDH and SNDH of the present invention are useful enzymes having preferable properties for the production of 2-keto-L-gulonic acid, as well as L-ascorbic acid. According to the production method of the present invention, the SDH and SNDH having such preferable properties can be produced in large amounts by genetic engineering.
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申请公布号 |
US6197562(B1) |
申请公布日期 |
2001.03.06 |
申请号 |
US19980118317 |
申请日期 |
1998.07.17 |
申请人 |
FUJISAWA PHARMACEUTICAL CO., LTD. |
发明人 |
NIWA MINEO;SAITO YOSHIMASA;ISHII YOSHINORI;YOSHIDA MASARU;SUZUKI HIROMI |
分类号 |
C12N9/04;(IPC1-7):C12N9/04;C12N9/02 |
主分类号 |
C12N9/04 |
代理机构 |
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主权项 |
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