| 摘要 |
PROBLEM TO BE SOLVED: To obtain the subject new enzyme preparation capable of specifically transferring a sulfate group from a sulfate group donor to an OH group at the 6-position of N-acetylgalactosamine 4-sulfate residue of glycosaminoglycan, suitable for production of a sulfated polysaccharide useful for a medicine, etc., function analysis, etc. SOLUTION: This new sulfate group transferase preparation specifically transfers a sulfate group from a sulfate group donor to an OH group at the 6-position of N-acetylgalactosamine 4-sulfate residue of glycosaminoglycan, transfers the sulfate group to chondroitinsulfuric acid A derived from a whale cartilage, chondroitinsulfuric acid C derived from a shark cartilage and dermatan sulfate derived from-swine skin but not to chondroitinsulfuric acid E derived from a squid cartilage, keratan sulfate derived from bovine cornea, heparan sulfate derived from bovine kidney and CDSNS-heparin, increases activity in the presence of Mn2+, Mg2+, Ca2+, NaCl, KCl, protamine, etc., inhibits the sulfate group from transferring to chondroitinsulfuric acid A by chondroitinsulfuric acid E. The preparation has uniformity and is obtained by extraction from an organism body such as squid cartilage, etc. |
