Methods and tissue culture media for inducing somatic embryogenesis, agrobacterium-medicated transformation and efficient regeneration of cacao plants

摘要

The present invention relates to improved methods of (i) inducing somatic embryogenesis from cacao tissue explants and (ii) regenerating cacao plants from somatic embryos. The invention further relates to cacao somatic embryos and plants obtained according to the methods of the invention. Novel tissue culture media adapted for use in the above-identified methods are also within the scope of the invention. The novel media of the invention include primary callus growth medium, secondary callus growth medium, embryo development medium, primary embryo conversion medium, secondary embryo conversion medium and plant regeneration medium.