A human lactoferrin produced by using an insect cell and method using the same

摘要

The producing process comprises Lactoferrin gene's producing recombined expression vector (pBacLf) (3) which is adjusted polyhedrin promoter in vector (pBacPAK) by combining transfer vector and remixed plasmid; producing recombined insect cell (Sf-Lf) (6) by cotransfection of the recombined revealed vector with help vector (pBacPAK) (6) at insect-cell in a culture medium; producing recombined insect virus from the recombined insect cell; and producing human Lactoferrin from the recombined insect-cell. In the invention, biological verifying method of recombined human Lactoferrin is measuring extinct rate of disease-causing germs after extracting recombined human Lactoferrin from recombined insect-cell and mixing it with disease-causing bacteria like Pseudomonas cepacia, Pseudomonas putida, Pseudomonas fluorescence, Salmonella typhimurium, E. coli.