Cell counting method and kit

摘要

There is provided a method for quantifying the number of target cells in a sample which comrises; a) applying the sample to a substrate, a surface of which is provided with a plurality of binding moieties each capable of binding specifically to the target cell so as to retain said target cells bound to said substrate surface, there being sufficient of said binding moieties to retain substantially all of the said target cells in the sample; b) washing the substrate and captured target cells to remove any extraneous non-specifically bound matter; c) lysing the retained target cells; and d) quantifying at least one component of the retained target cells to provide an indication of the number of retained target cells.