| 摘要 |
The present invention provides libraries of fusion nucleic acids each comprising nucleic acid encoding a nucleic acid modification (NAM) enzyme, a nd nucleic acid encoding a candidate protein. Also provided is a library of fusion polypeptides comprising a nucleic acid modification (NAM) enzyme and a candidate protein. A library of expression vectors is provided each comprisi ng (i) a fusion nucleic acid comprising a nucleic acid encoding a nucleic acid modification (NAM) enzyme, and nucleic acid encoding a candidate protein, an d (ii) an EAS. At least two of the candidate proteins are different. Preferabl y, the NAM enzyme is a Rep protein. Also preferably, the EAS is greater than 20 nucleotides in length. Similarly, preferred embodiments utilize fusion nucle ic acids comprising nucleic acids encoding presentation structures, nucleic aci ds encoding labels or nucleic acids encoding targeting sequences. The invention also provides libraries of nucleic acid/protein (NAP) conjugates each comprising a fusion polypeptide comprising a NAM enzyme and a candidate protein. The NAP conjugates also comprise an expression vector comprising a fusion nucleic acid comprising a fusion nucleic acid comprising a nucleic ac id encoding a NAM enzyme, a nucleic acid encoding a candidate protein, and an enzyme attachment sequence (EAS) that is recognized by the NAM enzyme. The E AS and the NAM enzyme are covalently attached. Libraries of host cells and methods of screening are also provided.
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