发明名称 |
Method for the stimulation of T cells having a desired antigen specificity |
摘要 |
T cells having a desired antigen specificity are stimulated by (a) introducing immortalizing genes into antigen-presenting cells in a manner permitting regulation of the expression and/or function of at least one of these genes to achieve conditionally immortalized antigen-presenting cells; (b) introducing a gene encoding the desired antigen into the immortalized cells in a manner permitting the antigen to be expressed after the expression and/or abolishment of the function of at least one of the immortalizing genes stops; (c) expanding the immortalized antigen-presenting cells by expression and/or functional activation of the immortalizing genes; (d) completing the proliferation of the immortalized antigen-presenting cells by stopping the expression and/or abolishing the function of at least one of the controllable immortalizing genes; (e) continuing the expression of the antigen; (f) adding leucocytic cells including T cells and cultivating the cell mixture to stimulate the T cells directed against the desired antigen; and (g) optionally purifying and isolating the stimulated T cells.
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申请公布号 |
US6194205(B1) |
申请公布日期 |
2001.02.27 |
申请号 |
US19980152653 |
申请日期 |
1998.09.14 |
申请人 |
GSF-FORSCHUNGSZENTRUM FUR UMWELT UND GESUNDHEIT GMBH |
发明人 |
STAEGE MARTIN;KEMPKES BETTINA;BORNKAMM GEORG W.;HAMMERSCHMIDT WOLFGANG;ZIMBER-STROBL URSULA;POLACK AXEL |
分类号 |
C12N15/09;A61K31/00;A61K35/14;A61P31/00;A61P31/12;A61P35/00;C12N5/0783;C12N5/10;(IPC1-7):C12N5/10 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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