| 摘要 |
In accordance with the present invention, there is provided a novel restriction endonuclease and its DNA obtainable from Helicobacter pylori CH4 (NEB#1236), hereinafter referred to as "HpyCH4IV", which endonuclease:(1) recognizes the nucleotide sequence 5'-ACGT-3' to in a double-stranded DNA molecule as shown below,5'-A↓CGT-3'3'-TGC↑A-5'(wherein G represents guanine, C represents cytosine, A represents adenine, T represents thymine and N represents either G, C, A, or T);(2) cleaves said sequence in the phosphodiester bonds between the A and C as indicated with the arrows; and(3) cleaves double-stranded PhiX174 DNA to produce 19 fragments, including fragments of 1036, 749, 397, 379, 371, 365, 362, 343, 282, 282, 268 base pairs, and 8 fragments smaller than 200 base pairs.
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