发明名称 |
Facile method for identifying regulated promoters |
摘要 |
A new method for the identification of useful promoters is disclosed. The method is capable of identifying bacterial promoters sensitive to a particular cellular insult and may be modified to identify promoters sensitive to herbicides and crop protection chemicals. Constructs comprising promoters upstream of a luminescent reporter genes are placed in transformed hosts. Transformants grown in liquid media to a predetermined growth stage and contacted with a cellular insult are assessed for regulatory region activity by measurement of the resulting change in bioluminescence. The method is able to identify promoters undetectable by standard methods.
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申请公布号 |
US6194159(B1) |
申请公布日期 |
2001.02.27 |
申请号 |
US19990449083 |
申请日期 |
1999.11.24 |
申请人 |
E. I. DU PONT DE NEMOURS AND COMPANY |
发明人 |
LAROSSA ROBERT ALAN;VAN DYK TINA KANGAS |
分类号 |
C12N1/21;C12Q1/68;(IPC1-7):C12Q1/68;C12N15/63;C07H21/04 |
主分类号 |
C12N1/21 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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