| 摘要 |
1. A cDNA sequence encoding mammaglobin polypeptide, or a derivative thereof having the analogue function. 2. A sequence derivative of claim 1, wherein comprises the sequence, complementary to SEQ ID NO: 1 or a derivative thereof. 3. The mammaglobin polypeptide with an amino acid sequence: or a derivative thereof. 4. The method for detecting the presence of breast cancer in a patient comprising detecting and/or quantitating the presence of a mRNA encoding a mammaglobin polypeptide of claim 3 in a sample from the patient, comprising incubating the sequence of claim 2 with the sample under conditions in which said mRNA can specifically hybridize with cDNA, wherein an elevated concentration of said hybridized complex above the concentration for a healthy individual indicates the presence of breast cancer cells. 5. The method for detecting the presence of breast cancer in a patient comprising detecting and/or quantitating the presence of a mRNA encoding a mammaglobin polypeptide in a sample from the patient, wherein said mammaglobin polypeptide comprises using the reverse transcription method , of said mRNA to obtain cDNA, amplifying the cDNA region by the polymerase chain reaction method using the two primers, complementary to the flanking regions of said c DNA region, and detecting the amplified cDNA region, wherein the elevated concentration of said amplified cDNA region above the concentration for a healthy individual indicates the presence of breast cancer cells. 6. The method of claim 5, the primers comprise: 7. A kit of claim 4, wherein comprises a sample comprising the sequence of claim 2 packaged in a container. 8. A kit of claim 5, wherein comprises two primers for the polymerase chain reaction packeged in a container. 9. A kit of claim 8, wherein two primers comprise: 10. A method for detecting the presence of breast cancer in a patient which comprises detecting the presence of a mammaglobin polypeptide of claim 3 in a sample comprising incubating an antibody to said mammaglobin polypeptide of claim 3 or to a mammaglobin epitope comprising at least five amino acids with said sample under conditions in which said antibody can bind with the mammaglobin polypeptide, and detecting the complex antibody-polypeptide, wherein wherein the elevated concentration of said complex above the concentration for a healthy individual indicates the presence of breast cancer cells. 11. The method of claim 10 wherein the antibody is a polyclonal antibody and the mammaglobin epitope comprises: 12. The method of claim 10 wherein the antibody is a monoclonal antibody and the mammaglobin epitope comprises: 13. A kit of claim 10 comprising an antibody to said mammaglobin polypeptide of claim 3 or the mammaglobin epitope comprising at least five amino acids packeged in a container. 14. The kit of claim 13 wherein said antibody is a monoclonal antibody and the mammaglobin epitope comprises: 15. The kit of claim 13, wherein said antibody is a polyclonal antibody and the mammaglobin epitope comprises: |
