| 摘要 |
PROBLEM TO BE SOLVED: To diagnose and detect arthritis, including discrimination thereof, in the early stage by measuring the concentration of melanotransferrin(MTF) in a biological sample by a method utilizing an antibody exhibiting high specificity to MTF. SOLUTION: First and second anti-MTF monoclonal antibodies are utilized in the measurement of concentration of MTF contained in articulation fluid or blood serum. An assay plate, where a microplate is coated with the first anti-MTF monoclonal antibody is used and a second biotin anti-MTF monoclonal antibody, is used as a second antibody in a specified sandwich type enzyme immunoassay for measuring the MTF concentration of articulation fluid of an arthritic patient. A reagent kit for use in this method is also provided. MTF in articulation fluid is significantly high in the case of arthritis and a significant difference appears in the distribution of MTF concentration in case of arthritis, arthritis rheumatica or traumatic injury of articulation.
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