INTERNALLY REFERENCED IMMUNOASSAY AND TEST DEVICE

摘要

A method of, and apparatus for, detecting the presence of an analyte in a sample, the method comprising: a) contacting the sample with a binding reagent having a binding specificity for the analyte and an analyte receptor located within a test zone to form a first complex comprising the binding reagent, analyte, and analyte receptor, the first complex providing a test signal; (b) contacting the sample with a reference ligand to form a second complex comprising the binding reagent, which further has a binding specificity for a reference ligand, and the reference ligand, the second complex providing a reference signal, wherein the reference ligand is located within a reference zone; whereby the ratio of the test signal to the reference signal defines the level of analyte in the sample, this level being independent of the quantity of binding reagent contacted with the sample.