New nucleic acid from phage phiCh1, used to create vectors for expressing proteins and polymers in halophilic Archaea

摘要

Isolated nucleic acid (I), containing at least one of 86 tabulated open reading frames (ORF), from the genome of phage phiCh1 of Natrialba magadii, at least one expression regulatory element for the ORF and/or the origin of replication (ori) of phi Ch1, is new. The genome of phi Ch1 is a combination of 48300 and 10198 base pair sequences (S1 and S2), both fully defined in the specification. Independent claims are also included for the following: (1) recombinant vector (RV1) containing at least one copy of (I); (2) recombinant vector (RV2) containing the ori of phi Ch1; (3) cell transformed with RV1 or RV2; (4) isolated polypeptide (II) encoded by (I); N. magadii cells free from the prophage of phi Ch1; (5) phage variants having lytic properties different from those of wild-type phi Ch1 and having genomic sequences at least 70 % homologous with the phi Ch1 genome; (6) use of phi Ch1 as gene transfer vector; and (7) use of halophilic Archaea for production of proteins and other polymers.