PRIMER EXTENSION MASS SPECTROSCOPY NUCLEIC ACID SEQUENCING METHOD

摘要

PCT No. PCT/GB94/02527 Sec. 371 Date May 10, 1996 Sec. 102(e) Date May 10, 1996 PCT Filed Nov. 17, 1994 PCT Pub. No. WO95/14108 PCT Pub. Date May 26, 1995A method of sequencing a target nucleic acid using primer extension mass spectroscopy to generate an observed mass spectrum, on which base calling cycles are carried out using calibrated inter-peak mass difference values such that: each called base allows mass calculation for the base-called peak in the observed mass spectrum; this calculated mass is then used as a further calibration point for subsequent rounds of base calling. A reaction mixture of all four base-specific chain extension nucleotides and four chain termination nucleotide analogues. A method of performing mass spectroscopy, which method comprises subjecting molecular ions which have been chemically charged in a predetermined manner to time-of-flight or Fourier transform mass spectroscopy.